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Beads were resuspended in 2x Sample buffer (2% SDS, 10% glycerol, 0.004% bromophenol blue, 0.125 M Tris-Cl, pH 6.8, 5% DTT) and boiled for 10 min at 95 C
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Concurrently, HIF-1 upregulates the expression of LDH and pyruvate dehydrogenase kinase (PDK), promoting the conversion of pyruvate to lactate and inhibiting the entry of pyruvate into TCA cycle, further shifting energy metabolism toward glycolysis (Cai et al., 2020)
(2025) discovered that Talaromyces purpurogenus , enriched in the gut of PCOS patients, produces the metabolite AT-C1, which suppresses the AhR signaling pathway and reduces IL-22 secretion, thereby inducing PCOS-like symptoms (69)
Improved glucose control Cagrilintide may help regulate blood sugar levels, which is particularly beneficial for individuals with type 2 diabetes
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