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To introduce mutations to c-MET, short gene fragments (100150 nt) containing mutations were synthesized (IDT Inc.), and the corresponding gene fragment in the wild-type c-MET was replaced by the synthesized gene fragment using HiFi DNA assembly enzyme (NEB)
Two receptors
Did my research for about two weeks before deciding to give it a shot
Hypokalemia (low potassium) This happens when rapidly increased red blood cells pull potassium from the blood
After 10 min, the reaction mixture was diluted to stop the reaction, and the production of LTB 4 was measured with the LTB 4 ELISA kit (Cayman Chemical)
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