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The final Real-Time PCR protocol was adjusted for use in the IQ5 PCR Thermal Cycler instrument (BioRad, Minnesota, USA) and used the RH92600 gene as a reference gene (primers as previously reported) [14]

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At 4 mg, the relative risk of diarrhea was 1.64 compared to placebo, with a 95% confidence interval of 0.77 to 3.49