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Isopropyl -D-1-thiogalactopyranoside (IPTG), tris(hydroxymethyl)aminomethane (Tris), tetramethylethylenediamine, glycerol and acrylamide (30%, 37.5:1 acrylamide:bis-acrylamide) were products of AppliChem GmbH (Darmstadt, Germany)