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The enhanced cocktails, CILy (CHIR+IDE1+Ly) and CIP (CHIR+IDE1+PD), significantly increased the expression of DE markers SOX17 and FOXA2, with CIP showing the highest efficiency, comparable to the traditional CA method

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On the other hand, glycation of arginine and lysine residues can block the sites of the proteases responsible for the protein digestion ( Most studies addressing this issue have been performed in vitro , using nonenzymatic glycation of proteins of known structure, followed by their enzymatic degradation

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