Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific, 15-340-154) containing lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail and homogenized using a bead mill (VWR) for 10 s
[14] A subtherapeutic LDL-C response could reflect non-adherence with the injection schedule, poor subcutaneous absorption due to injection technique, or a rare neutralizing antibody, not glutathione co-administration
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