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These three assays were carried out as previously described [17] with some modifications
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Eligible participants were adults without diabetes with a BMI of at least 30 kg/m, or at least 27 kg/m with hypertension or dyslipidaemia
Sections were counterstained with haematoxylin (CS700, Dako-Agilent or RE7107-CE, Leica) and mounted with Mounting Medium, Toluene-Free (CS705, Dako-Agilent) using a Dako CoverStainer
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