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When cells reached 80% confluence, the monolayer was rinsed 3x with PBS and cultured in phenol red-free MEM without additives (no FBS, L-glutamine, ITS, or estradiol)
10.1093/toxsci/kfy297 85 ZmijewskiJ
If a vial is multi-dose, follow the label or pharmacist instructions for how long it can be used after opening
The oral capsule format eliminates the reconstitution step required for lyophilized vials
They are oral, regulated as supplements rather than injectables, and considered low risk
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