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Contrary, NP4 showed a cytotoxic effect in the very low micromolar range (143B: IC 50 = 6.6 M, K7M2: IC 50 = 2.1 M) (drug-response curves: Fig

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Using co-immunoprecipitation (CO-IP), the study confirmed that FOXO4-DRI prevents FOXO4 binding to p53, facilitating phosphorylated p53 nuclear exclusion, which triggers BAX upregulation and cleaved caspase-3 activation driving selective apoptosis of senescent endothelial cells