(Wikipedia) In senescent cells (cells that have permanently exited the cellcycle, accumulate damage, and secrete inflammatory factors), FOXO4 expression goes up and helps bind p53 in the nucleus, preventing p53 from driving those cells into apoptosis
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RNA extraction and real time quantitative PCR Total RNA was isolated from liver tissue using Trizol (Roche, Mannheim, Germany) in accordance with manufacturers instructions and was converted to cDNA using a previously described method 5
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