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Genetic variability in GLP-1 receptor is associated with inter-individual differences in weight lowering potential of liraglutide in obese women with PCOS: a pilot study

Sunil Badve, a kidney specialist and professorial fellow at The George Institute for Global Health in Sydney, Australia

After cleavage with His-TEV to remove the His-MBP tag, the eluted fraction (SNX1-His-SUMO-SNX2a) was further loaded onto a Ni-NTA column and washed with buffer containing 20 mM HEPES (pH 7.4), 200 mM NaCl, and 40 mM imidazole to remove unbound samples

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