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Nuclear NF-B binds B response elements in promoter regions of pro-inflammatory genes including IL-6, TNF-, IL-1, COX-2, and iNOS, initiating transcriptional upregulation [86]
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Site-saturation libraries targeted six sets of three proximal residues facing the ligand-binding pocket for combinatorial mutagenesis with degenerate NNS codons, resulting in 48,000 possible variants
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