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The membranes were blocked with Starting Block T20 PBS Blocking Buffer (Thermo Fisher Scientific, Inc.) and probed overnight at 4C with the primary antibodies diluted with 10% Blocking Ace (DS Pharma Biomedical) in PBS containing 0.1% Tween-20

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Detection strategies increasingly target metabolites that persist longer than parent compounds

Progress in this area is further hindered by limited funding and commercial incentives

Physical interactions, pathways, and genetic interactions were generated, and datasets relevant to ovarian failure and the prosurvival signaling network were collected ( LC-MS Scheduled MRM Analysis for Target Protein Determination We next performed an LC-MS MRM scan of the isotopically labeled peptides for relative quantitation of these selected proteins to further determine the functions of the selected proteins in the targeted signaling pathways