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These hormones are secreted from intestinal mucosal cells, and GLP-1 can be detected in the circulation minutes after oral ingestion of nutrients
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Immunohistochemistry of the brainstem Sections were washed (3 5 min) in Tris buffered saline (TBS) containing 0.3% Triton X-100 (TBST buffer), endogenous peroxidases were deactivated by incubating in peroxidase block (hydrogen peroxide) for 10 min with gentle agitation, and sections were rinsed with TBS and incubated with target retrieval solution (Dako, Glostrup, Denmark) for 30 minutes at 80 C and washed again with TBST (3 5 min)
Patients must adhere to storage instructions provided to ensure that the medication remains effective throughout its shelf life
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