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Description

Baker WL, Smyth LR, Riche DM, Bourret EM, Chamberlin KW, White WB

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To increase activity, we transitioned to PACE using a more stringent circuit, replacing the T7 RNAP promoter with the weaker T3 RNAP promoter 50 and adjusting RBS strength in front of gIII to reduce transcriptional activation following RNAP liberation (Extended Data Fig

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