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Quantitative PCR was performed via the use of primers for the human target genes involved in iron homeostasis, ferroptosis, and inflammation processes (Supplementary Tables 1 and 2) with GoTaq qPCR Master Mix (Promega) via a Quant Studio 5 PCR System (Thermo Fisher Scientific)
Birari L, Wagh S, Patil KR, Mahajan UB, Unger B, Belemkar S, et al
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ACSA-2 + astrocytes (Miltenyi Biotec, 130-097-678) were isolated using manual MACS according to the manufacturers instructions
HPA (hypothalamus-pituitary-adrenal) axis support: With your FM practitioner, you can assess for HPA axis dysregulation
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