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To construct expression vectors for the secretion of Exe4, double-stranded DNAs of Exe4 fused with signal peptides (SPs) and furin-cleavable sequences were synthesized (Azenta) and inserted into a CAG promoter-containing pCAG backbone vector using the In-Fusion HD cloning kit (Takara Bio)

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With a personalized treatment plan involving oral and topical medications, along with Pico laser and medical peels, she experienced a considerable reduction in pigmentation and an improvement in skin tone

Drizzle olive oil and add one pinch of salt and black pepper