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[5] The ratios are inconsistent across species, which is itself telling, but in every case the subcutaneous dose required was substantially higher than the intravenous one

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This increase in ATP shuts down membrane ATP-sensitive K + channels, depolarizing plasma membrane, and activating voltage-gated calcium channels ( 2+ influx seems to be necessary for the fusion of insulin granules with the plasma membrane, ending up in its secretion (Olofsson et al., 2002)