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Immunocytochemistry Anti-GFP (1:1000, Abcam) antibody was applied in blocking solution (PBS, 0.05% BSA, 2% FBS, 1% Triton X-100, and 0.1% saponin) for 2 h at room temperature
The cost is affected by the number of injections necessary each week or month
2018. Therapeutic potential of NAD-boosting molecules in vivo
Even under these conditions, NADH-fuelled CI respiration remained higher at ZT14 than ZT2 (Fig
The temperature should be around 140150F (hot, but not scalding)
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