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Breastfeeding mothers are often more conscious of the environmental toxins they may encounter in daily life
For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)
SubQ allows less frequent dosing (every 34 days) while maintaining therapeutic dermal concentrations
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