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Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100
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10.3390/cancers9090116 24 Noren HootenN.PachecoN
doi:10.1016/j.nutres.2016.03.010 Dzupina A, Pella D, Zenuch P, et al
Muscle is metabolically active tissue
This is usually only recommended for individuals on high maintenance doses who wish to minimize the volume of the injection
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