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Samples were vortexed and left at 20 C for 1 h, centrifuged for 10 min at 21,130 g and 4 C and transferred to glass vials for their analysis by LCMS

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Reporting suspected adverse reactions : If you experience a suspected side effect from an unlicensed product, please report it via the MHRA Yellow Card scheme (available at yellowcard.mhra.gov.uk)