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Allison Steele, a bariatric physician at HonorHealth, to uncover the key nutrition choices that can make or break your progress
This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]
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