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The protocol was straightforward: 1000 mg glutathione mixed in 20cc saline, delivered slowly over 15 minutes every 7-10 days
Afterward, different amounts of the hydrogel powder samples were dispersed in 5 mL 100 M DPPH (2,2-diphenyl-1-picrylhydrazyl) ethyl alcohol solution to form different concentrations of PTK (0.5, 1, 2, 3, 5, 10 mg/mL) and PMI (10 mg/mL) solutions
The RNA, both input and immunoprecipitated, was extracted and subsequently subjected to either next-generation sequencing (NGS) or qRT-PCR
By elevating NAD+ and activating SIRT1, 5-Amino-1MQ promotes the proliferation and differentiation of muscle stem cells into fusion-competent myoblasts, facilitating effective muscle repair and regeneration
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