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Potassium/proton antiport system of Escherichia coli
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Erythrocytes were lysed twice by adding DEPC-treated H 2 O and NaCl (8.8%), followed by centrifugation (450 x g, 6 min, 4C, Universal 320 R, Hettich, Tuttlingen, Germany)
Inserting the filter into the bottle can be a bit tricky, but once you get the hang of it, its pretty easy to use
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