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Then, 15 g of protein was mixed with NativePAGE sample buffer (Invitrogen), Coomassie G-250 and protease inhibitor, loaded into the lanes of a 312% Bis-Tris NativePAGE gel (Invitrogen) and run according to the manufacturers instructions
HRE, hypoxia response element
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Toxicol Pathol 32:619642 Wheelock , Wheelock C (2013) Trials and tribulations of omics data analysis: assessing quality of SIMCA-based multivariate models using examples from pulmonary medicine
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