cAMP accumulation assay FlpInCHO-hGLP-1R and FlpInCHO-pGLP-1R cells were diluted to the density of 6 10 5 cells/ml and INS-1(832/13) cells to 2 10 6 cells/ml in stimulation buffer [phenol-free DMEM containing 5 mM HEPES, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) and 0.1% (w/v) BSA], and cAMP detection was carried out as described previously 48
Common mild side effect may include soreness or redness at injection site, mild diarrhea, nausea, headache, and itching
However, skipping multiple weeks or months may reset your steady-state levels and cause appetite return, potentially requiring dose re-titration
Results: Nausea, vomiting, diarrhea, and constipation were the most frequently reported GI adverse events, generally dose-dependent and transient
Wait at least 30 minutes before consuming any food, beverages, or other medicines