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This can be prevented by derivatizing GSH with the alkylating agent N -ethylmaleimide (NEM) to form GS-NEM before acid deproteination, followed by back-extraction of excess NEM from the deproteinized samples with dichloromethane
Proc Natl Acad Sci USA 86: 471475
So amino acids gets used up to make other peptides and other proteins that you need
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