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The coupling reaction was initiated by incubation at 25 C for 1 h and was followed by the addition of Apyrase (New England BioLab) to catalyze the hydrolysis of GDP overnight at 4 C

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Patients without Neurofibromatosis type 1 (NF1) tend to have better outcomes than those with NF1-associated MPNST, as NF1-related tumors are more likely to be multifocal, high-grade, and resistant to therapy (Evans et al., 2021)

Thus the rapid reimport hypothesis explains both sets of data

The impact of this launch can be assessed through prescription trends, adoption rates in clinical practice, and its role within existing diabetes management frameworks