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GSH is made exclusively in the cytoplasm of cellsthe water-soluble compartment of the cell

The linearity of the modified GPx-DTNB assay was assessed by comparing its results to those of the original GPx assay method (Rotruck et al

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Moreover, it is more stable, more effective, and able to reduce disulfide bonds at lower pHs if compared to DTT and odorless oppositely to 2-ME 29,30,31

in its active form (thiamine diphosphate), it is an essential cofactor for enzymes in glucose and amino acid catabolism, helping convert nutrients into ATP and supporting normal neuronal function