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The shRNA sequence (TRCN0000322995) targeting ATG10 was designed according to MISSION shRNA Plasmid DNA (MERCK) and synthesized and joined the FastDigest BshTI/FastDigest EcoRI linearized (Thermo Fisher) pLKO.1-GST DAF vector with T4 DNA ligase (EL0011, Thermo Fisher)
10.3945/ajcn.110.009340 117 ZhangZ.AtwellL
Hao, Y., Zhao, W., Zhang, H., Zheng, W
In db/db mice and HG-treated TECs, ectonucleoside triphosphate diphosphohydrolase 5 overexpression inhibited apoptosis to preserve TECs via regulating the protein N-glycosylation rate in ER (83)
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