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Cells were then harvested by trypsinization, washed once with phosphate-buffered saline (PBS) and re-suspended in 300 l phenol red-free DMEM with 0.2% bovine serum albumin
Reactive oxygen species including hydrogen peroxide are also involved in other cellular processes, such as mitochondrial function and growth factor-mediated cell signaling, thus GPx may also affect such aspects of cellular function 60
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However, further metabolomic and proteomic studies are still needed to investigate the mechanisms of relevant microbial metabolism-host interactions
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