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SuperSignal Chemilluminscent substrate HRP system (Peirce, Thermo Fisher Scientific), was added to the membranes for 5 min, and then exposed to x-ray CL Xposure films (Thermo Fisher Scientific) and imaged using Photons Developer Instrument
Additionally, the newly synthesized compounds significantly reduced LPS-induced production of ROS and pro-inflammatory cytokines (IL-6, TNF-, and NO) in RAW 264.7 macrophages
Based on their subcellular localization, HSP70 family members can be divided into cytosolic/nuclear HSP70s, including HSPA1, HSPA1L, and HSPA2
doi: 10.1158/0008-5472.Can-20-0600 90 AlessioMMariaFIolandaSMariaCVRosannaGAnnaDGet al
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