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Mechanical stimuli such as shear stress and cyclic stretching help mimic the physiological kidney environment and promote functional maturation

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The samples were load onto 10 kDa spin columns to remove the free biotin picolyl azide using a buffer containing 50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 0.5 mM EDTA, 0.1% Triton X-100, 0.1% SDS, and 0.5% NP40