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The amount of free ENZ in the supernatant was measured using spectrophotometry at a wavelength of 234 nm, using a calibration curve prepared with a standard ENZ solution

IV drip therapy is a medical procedure that involves delivering a mixture of fluids, vitamins, minerals, and other nutrients directly into the bloodstream through a vein using an IV cannula

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The authors intended to change the ionic environment of the fish muscle, by using this chelating agent to scavenge for calcium ions, and therefore promote a potentially hypoallergenic fish (reduced content of Ca 2+ -bounded parvalbumin in the muscle)