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The supernatant was passed through a mouse anti-human AIM IgG monoclonal antibody (CL7)-immobilized HiTrap NHS-activated HP column and the protein was eluted with 0.1 M glycine-HCl (pH 2.5) and neutralized with 1 M Tris-HCl (pH 8.5)
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Cell Death Discovery 8(1):475 Mustafa M, Ahmad R, Tantry IQ, Ahmad W, Siddiqui S, Alam M et al (2024) Apoptosis: a comprehensive overview of signaling pathways, morphological changes, and physiological significance and therapeutic implications
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