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Cell Death Discovery 7(1):236
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Proteins were precipitated by adding four volumes of ice-cold acetone and subsequent centrifugation (21.000 g , 20 min, 4 C)
Hoechst 33342 staining of the bovine blastocyst (Figure 6A) was also applied to assess the developmental potentials of cryopreserved bull semen with the cell number/blastocyst of each group analyzed
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