This type of study is important for several reasons: it tells us how long the peptide remains active in the body, what breakdown products are formed (which could themselves have biological activity or be used as markers in drug testing), and helps explain why AOD9604 needs to be administered in certain ways to remain effective
In the single source study, AHK-Cu stimulated the proliferation of cultured human DPCs and the elongation of isolated human hair follicles at very low concentrations (1010 M), raised the Bcl-2/Bax ratio and lowered cleaved caspase-3 and PARP though the drop in apoptotic-cell count itself was not statistically significant [1]
These protocols form the foundation for current therapeutic dosing recommendations
It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile

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