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Detachment of cytochrome c from cardiolipin might also be triggered by increased cytosolic calcium, which weakens the electrostatic interaction between cytochrome c and cardiolipin and further generates ROS via MPT

However, it can be difficult to maintain these levels, especially as you get older, live under constant stress, or have an ongoing health problem

Cells were collected, pelleted, washed with 1 PBS and finally 250 l of 1 PBS buffer containing 10% FBS and EDTA (0.1% v/v) was added for flow cytometry

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The role of oxidative stress in the COVID-19 disease may involve recognition of the viral S-protein by angiotensin converting enzyme-2 (ACE2) receptor and pattern recognition receptors like toll-like receptors 2 and 4, and activation of transcription factors like nuclear factor kappa B, that subsequently activate nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) expression succeeded by ROS production (Kozlov et al., 2021)