In the following steps of elongation, 3-ketoacyl-CoA reductase, 3-hydroxyacyl-CoA dehydratase and trans-2,3-enoyl-CoA reductase catalyze reduction, dehydration and reduction, respectively
Intracellular production of OH from H 2 O 2 is driven by the Fenton reaction, shown in Reaction 3 (43, 44, 48): As the most reactive known ROS, OH reacts with nearly all intracellular building blocks and macromolecules (amino acids, nucleic acids, phospholipids, sugars, proteins, RNA, DNA, lipids, polysaccharides)
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For most organelles, the average fraction of half-tryptic peptides was similar or slightly lower for in-cell LiP-MS than for in-lysate LiP-MS, reflected also in the comparison for all peptides from all organelles (Appendix Fig