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Detection of percentage positive cells was based on a threshold, typically set according to the average pixel intensity of an internal negative control or an unstained secondary antibody-only control sample, unless specified
Nature (2019) 572(7769):32934
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Likewise, substitution of the corresponding residue Asp433 with asparagine (D433N) in EcGGT abolished its transpeptidase activity, and the hydrolytic variant evolved retained almost 82% activity (Suzuki et al., 2004c)
Int J Biochem Cell Biol (2009) 41:184654
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