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After pre-blocking, pancreas samples were stained with primary antibodies in 5% v/v goat serum

Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2

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This combination could be particularly effective for people with stubborn visceral fat

For our studies, chondrocytes were expanded in vitro in GM and incubated at 37C with 5% CO 2