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A total of 250 ng of target DNA was used per reaction
Nature 425, 191196
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Some experimental and clinical evidence also suggests that the early administration of intravenous L-carnitine could further improve survival in severe VHT
Note that 2-PAM has an affinity for the acetylcholine anionic binding site on cholinesterase (right middle panel), which could explain the oxime related worsening of cholinergic side effects when 2-PAM is administered prior to the onset of action of atropine in the setting of organophosphate poisoning, by transiently preventing nerve-agent free cholinesterase from interacting with acetylcholine
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