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Although NMR using fluorescent biosensors or optical reporter tags can provide a valuable perspective for identifying the biochemistry of living cells, it, like the MS based approach, is of limited use for in situ tracking of metabolic processes (Lerche et al., 2015), due to the inherent physicochemical properties of small and hydrophilic metabolites, such as low ionisation efficiency and high detection limits (Chalcraft et al., 2009

Upon ligand binding, the nanoparticle-receptor complex is internalized via clathrin-coated vesicles to be transported across the BBB and subsequently released by exocytosis into the brain parenchyma 119,120