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After washing three times with TBST, membranes were incubated with primary antibodies at 4 overnight

Its capacity to neutralize reactive oxygen species (ROS) helps protect nerve cells from oxidative stress, a key factor in aging and neuro-degenerative conditions (10, 43)

For intracellular staining of hexokinase-1, cells were stained for extracellular markers, fixed in 4% FA at RT, and stained with Anti-Hexokinase 1 (Abcam ab184818) diluted 1:100 in PBS 0.1% Tween-20 at RT

Funding This research was supported by the Beijing Natural Science Foundation (6212009), and the Reform and Development Project of Beijing Academy of Agricultural and Forestry Sciences (XMS202411)