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Co-culture experiments with PC3 WT and GGT1 + cell lines were performed using 6-well (24 mm) PET inserts with 0.4 m pores (VWR, 76313-902 or Corning, 354570)
Armstrong et al., 2018)
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use within ~30 days TB-500 35.646.4F (28C)
This way it gets largely recycled so even if you dont have any B12 in your diet, it can take years for deficiency to develop (Temova Rakua et al., 2022)
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