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Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18
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The recommended volume is 2.0 mL, which creates a 2.5 mg/mL concentration
doi: 10.1016/j.fct.2007.06.001 5 AkinmoladunA
These alterations result in a significantly extended half-lifeup to 120 times longer than native IGF-1due to reduced binding affinity to insulin-like growth factor-binding proteins (IGFBPs)
Promotes detoxification
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