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Since mouse microbiome profiles often highly correlate with body weight and fat composition, this study was designed to test whether the cecal microbiomes of DIO mice treated with NNMTi and LD were comparable to the microbiomes of age-matched lean counterparts and distinct from microbiomes of DIO mice maintained on a high-fat Western diet (WD) or subjected to LD switch alone

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(Fortified is important because it ensures that thiamine levels are adequate.) Again, because excess B vitamins can be excreted, toxicity is extremely unlikely to be incurred

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