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Step 2: Use it on calm nights first

Briefly, cardiomyocytes were treated with PQQ as above and were then incubated for 20 min with a JC-1 working solution at 37 C, followed by two washed using JC-1 staining buffer

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An effect of one electron oxidation by oxidants may result in the modification of protein-SH groups and consequently the formation of thiyl radicals (Protein-S ) and protein sulfenic acid (Protein-SOH) (Valko et al

The relevance of this enzyme is illustrated by the fact that global SOD2 deficiency leads to neonatal death in mice (Huang et al., 1997)