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Leaky synapses: regulation of spontaneous neurotransmission in central synapses
The eluted sample was quickly neutralized by addition of 1/10 volume of 1 M Tris pH 8.0 and further polished on a size-exclusion chromatography column (Superdex 75, GE healthcare) pre-equilibrated with phosphate-buffered saline (PBS, pH 7.4)
they only tested mitofusin-2 levels, an indicator for mitochondrial activity, which was unaltered 259
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